Ebe mmepụta ihe: mmepụta na 100,000-klas dị ọcha ogbako
Ngwa akụrụngwa mmepụta: polystyrene dị elu (GPPS)
Usoro mmepụta: Nhazi ngwaahịa dị mma, ihe nlereanya zuru oke, nhazi nkenke, enweghị mgbakwunye kemịkal. Mejuputa nyocha nke anọ nke nyocha onwe onye, nyocha patrol, nyocha zuru oke na nyocha nke enweghị usoro, na àgwà ahụ kwụsiri ike.
Ọgwụgwọ dị n'elu: ọgwụgwọ TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment
Usoro TC, dabara maka ọdịbendị nke sel ndị na-akwado ya
N'iji ọgwụgwọ plasma pụrụ iche na-ekpo ọkụ gas, elu nwere ike ịnọgide na-ebu ụdị abụọ nke otu n'otu n'otu ruo ogologo oge, ụgwọ dị mma na ụgwọ na-adịghị mma, jide n'aka na ntinye nke cell na-ejikọta ya na nke ọma. TC nwere mma mmachi na mgbasawanye karịa ihe ndị yiri TC ndị yiri ya mgbe ejiri ya mee ihe maka mkpụrụ ndụ endothelial, hepatocytes na omenala neuronal cell, ma nwee ike nweta arụmọrụ adhesion cell kacha mma. Meeju omenala nke sel ndị na-akwado ọkwa dị elu.
Usoro ọgwụgwọ TC kwalitere, dabara adaba maka ọdịbendị cell nwere nnukwu nrube isi
Ọdịbendị nke anụ ahụ dị elu, ma e jiri ya tụnyere ngwaahịa TC-ọkọlọtọ, elu TC kwalitere nwere ikike ịkwalite adhesion cell na ndọtị, gbasaa ọnụ ọgụgụ sel ngwa ngwa, enwere ike iji ya gbasaa mkpụrụ ndụ na-achọsi ike, dị ka mkpụrụ ndụ isi ma ọ bụ sel nwere mmetụta, dị ka yana sel azụlitere n'okpuru ọnọdụ uto amachibidoro (n'efu ma ọ bụ serum-ebelata), na-abawanye ọnụ ọgụgụ sel na-abawanye ngwa ngwa, na-akwalite mgbakwunye cell na ndọtị, ma enwere ike ịchekwa ya na ụlọ okpomọkụ yana ndụ shelf nke afọ 2 yana ọtụtụ ụdị. ngwa.
Ultra-ala adịgide adịgide usoro, adabara omenala nke nkwusioru sel
Na-ekpuchi polymer amphiphilic pụrụ iche n'elu arịa omenala, n'ihi na hydrophilicity siri ike nke ogige a, ụmụ irighiri mmiri amphiphilic nwere ike ịmịnye mkpụrụ ndụ mmiri iji mee mgbidi mmiri, nke mere na mkpụrụ ndụ, protein ụmụ irighiri ihe na nje bacteria enweghị ike itinye ya na arịa omenala. , ya mere o nwere njirimara nke ultra-low cell adhesion.
Enwere ike ịzụlite ya na nkwusioru ihe karịrị ụbọchị iri na ise. Mgbe anwalechara, ọnụego nnabata cell nke AMA ultra-low adsorption bụ ihe na-erughị 2% na omenala nkwusioru maka ụbọchị 21, nke yiri nsonaazụ nke akara mba ụwa ama ama.
A na-eji ya na-azụlite mkpụrụ ndụ sphere nwa ebu n'afọ, mkpụrụ ndụ na-agba ọbara na mkpụrụ ndụ ndị ọzọ dị mkpa na-eto eto na omenala nkwusioru, ma nwee ike ịkwalite ọdịbendị nke 3D spheroid cell na organoids, ma nwee ihe mgbochi-adhesion maka mkpụrụ ndụ viscous siri ike.
Collagen ụdị l ọgwụgwọ elu
A na-ahụ ụdị Collagen I n'ọtụtụ anụ ahụ na akụkụ ahụ, yana ọkwa kachasị elu dị na dermis, akwara na ọkpụkpụ. Ọ bụ protein nhazi ihe dị mkpa nke na-akwado sel na anụ ahụ dum, ma bụrụkwa ihe dị mkpa maka ịkwalite uto cell. Na omenala vitro, collagen na-arụ ọrụ dị mkpa na adhesion, morphogenesis, uto, ịkwaga na ọdịiche nke mkpụrụ ndụ dị iche iche.
Ngwa akọwapụtara gụnyere:
Na-akwalite adhesion cell na ịgbatị
ọnụ ọgụgụ cell na-agbasa ngwa ngwa
Omenala enweghị ọbara ma ọ bụ mbelata ọbara
Cell Adhesion Assay
Melite omenaala sel nke izizi
Nonpyrogenic, Nonendotoxicity, DNase/Rnase n'efu, echekwara na 2-8 Celsius, yana ndụ nchekwa nke ọnwa 6
Ịgba ọgwụ mgbochi mmiri
Poly-D-lysine mkpuchi elu ọgwụgwọ
Poly-D-lysine (PDL) bụ ihe sịntetik nke nwere ike ịkwalite cell adhesion na protein adsorption site n'ịgbanwe ụgwọ elu nke mkpụrụ osisi omenala.Na mgbakwunye na ịkwalite adhesion cell, poly-D-lysine-mered surfaces na-akwado mmepụta neurite ma melite ọganihu. Na-adị ndụ nke ọtụtụ Central ụjọ usoro (CNS) bụ isi cell omenala.Ebe PDL bụ a sịntetik molekul, ọ gaghị akpali ndị ndu ọrụ nke omenala mkpụrụ ndụ, ma ọ bụ ọ gaghị ewebata adịghị ọcha ebu site eke polymers.
Ngwa akọwapụtara gụnyere:
Adhesion na ndọtị nke sel dị iche iche
Ọdịiche nke cell na mpụta neurite
Adhesion nke ahịrị sel ebugharị ngwa ngwa
Mmụba nlanarị nke omenala neuronal nke mbụ
Omenala enweghị ọbara ma ọ bụ mbelata ọbara
Ọnọdụ nchekwa: N'okpuru ọnọdụ akọrọ na 2 ° C ruo 8 Celsius, ndụ nchekwa bụ afọ 2
Oge nzipu: Jul-08-2023