Deegaanka wax-soo-saarka: wax-soo-saarka 100,000-fasalka aqoon-is-weydaarsi nadiif ah
Wax soo saarka ceeriin: polystyrene tayo sare leh (GPPS)
Habka wax-soo-saarka: Naqshadaynta alaabadu waa mid heersare ah, qaabku waa dhammaystiran yahay, samaynta saxda ah, wax kiimiko ah oo lagu daray ma jirto. Si adag u dhaqan gelinta afarta kormeer ee is-baaritaanka, kormeerka roondada, kormeerka buuxa iyo kormeerka aan tooska ahayn, tayadana waa mid deggan.
Daaweynta dusha sare: TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment
Taxanaha TC, oo ku habboon dhaqanka unugyada ku dheggan
Isticmaalka daaweynta plasma gaaska vacuum gaar ah, oogada waxay sii wadi kartaa in ay qaadaan laba nooc oo kooxo ah si isku mid ah muddo dheer, lacag wanaagsan iyo lacag taban, hubi in u hoggaansanaanta unuggu uu yahay mid isku mid ah oo xasilloon. TC waxay leedahay adhesion iyo faafin ka wanaagsan sagxadaha TC ee la midka ah marka loo isticmaalo unugyada endothelial, hepatocytes iyo dhaqanka unugyada neerfaha, waxayna gaari karaan waxqabadka adhesion unugga ugu fiican. Ku qanac dhaqanka unugyada u hoggaansan heerka sare.
Taxanaha la wanaajiyey ee TC, oo ku habboon dhaqanka unugyada leh shuruudaha u hoggaansanaanta sare
Daaweynta dhaqameed horumarsan, marka la barbar dhigo alaabada TC-da ee caadiga ah, dusha sare ee TC-du waxay awood u leedahay inay kor u qaaddo adhesion unug iyo fidinta, si degdeg ah u ballaariso tirada unugyada, waxaana loo isticmaali karaa in lagu faafiyo unugyo dalbanaya, sida unugyada asaasiga ah ama unugyada xasaasiga ah, sida sidoo kale unugyo lagu dhaqay xaaladaha korriinka ee xaddidan (serum-free ama serum-lagu dhimay), waxay kordhiyaan tirada unugga ee degdega ah u fidaysa, waxay kor u qaadaan ku-xidhnaanta unugga iyo kordhinta, waxaana lagu kaydin karaa heerkulka qolka oo leh nolol shelf oo ah 2 sano iyo kala duwanaansho ballaaran. codsiyada.
Taxane xidhidh-hooseeya , oo ku habboon dhaqanka unugyada laalidda
Daboolida polymer amphiphilic gaar ah dusha markabka dhaqanka, sababtoo ah hydrophilicity gaar ahaan xooggan ee xaruntan, molecules amphiphilic waxay nuugi kartaa molecules biyaha si ay u sameeyaan derbiga biyaha, si unugyada, molecules borotiinka iyo bakteeriyada aan lagu dhejin karin weelka dhaqanka. , sidaas darteed waxay leedahay sifooyinka adhesion unug ultra-hoose.
Waxa lagu dhaqan gelin karaa ka joojinta in ka badan 15 maalmood. Imtixaanka ka dib, heerka ku dheggan unugga ee AMA ultra-hoose adsorption ayaa ka yar 2% dhaqanka hakinta muddo 21 maalmood ah, taas oo la mid ah natiijada astaanta caalamiga ah ee caanka ah.
Waxaa loo isticmaalaa in lagu beero unugyada sphere ee uurjiifka, unugyada spheres dhiigga iyo unugyo kale oo u baahan in ay koraan in dhexdhexaad dhaqanka ganaax, iyo kor u qaadi kartaa dhaqanka unugyada 3D spheroid iyo organoids, oo uu leeyahay sifooyinka anti-adhesion ee unugyo xoog viscous.
Nooca Collagen l daaweynta dusha sare
Nooca Collagen I waxa laga helaa unugyada iyo xubnaha intooda badan, oo leh heerarka ugu sarreeya ee laga helo maqaarka, seedaha iyo lafaha. Waa borotiin qaabdhismeed lama huraan ah oo taageera unugyada iyo unugyada oo dhan, sidoo kale waa matrix muhiim u ah horumarinta korriinka unugyada. Dhaqanka vitro-ga, kolajku wuxuu door muhiim ah ka ciyaaraa adhesion, morphogenesis, koritaanka, socdaalka iyo kala soocida unugyada kala duwan.
Codsiyada gaarka ah waxaa ka mid ah:
Waxay kor u qaadaa ku dhegganaanta unugga iyo kala bixinta
tirada unugyada oo si degdeg ah u fidaya
Serum-free ama dhaqanka serum-yaray
Baaritaanka Adhesion Cell
Hagaajinta badbaadada dhaqanka unugyada aasaasiga ah
Nonpyrogenic, Nonendotoxicity, DNase/Rnase bilaash ah, lagu kaydiyay 2-8°C, oo leh nolol shelf oo ah 6 bilood
Nadiifinta shucaaca
Daaweynta dusha dahaarka leh ee Poly-D-lysine
Poly-D-lysine (PDL) waa isku-darka synthetic kaas oo kor u qaadi kara xajinta unugyada iyo xayeysiinta borotiinka iyadoo la beddelayo kharashka dusha sare ee substrate-ka dhaqanka.Marka lagu daro kor u qaadista adhesion-ka unugyada, dusha sare ee poly-D-lysine-daaweeyay waxay taageertaa koritaanka neurite waxayna hagaajinaysaa koritaanka neurite. badbaadada badan oo ah habdhiska dhexe ee neerfayaasha (CNS) dhaqamada unugyada aasaasiga ah. Maadaama PDL ay tahay molecule synthetic, ma kicin doonto dhaqdhaqaaqa bayoolojiga ee unugyada dhaqanka, mana soo bandhigi doonto wasakh ay qaadaan polymers dabiiciga ah.
Codsiyada gaarka ah waxaa ka mid ah:
Adhesion iyo fidinta unugyo kala duwan
Kala soocidda unugyada iyo soo bixidda neurite
Ku dheggan xadhkaha gacanta ee degdega ah ee la kala beddelay
Badbaadada dhaqamada asaasiga ah ee neerfaha
Serum-free ama dhaqanka serum-yaray
Xaaladaha kaydinta: Xaaladaha qalalan ee 2°C ilaa 8°C, cimriga shelf waa 2 sano
Waqtiga boostada: Jul-08-2023