Malo opangira : kupanga mu 100,000-kalasi yoyera yophunzirira
Zopangira zopangira: polystyrene yapamwamba (GPPS)
Njira yopangira: Kapangidwe kazinthuzo ndi kokongola, mtunduwo ndi wathunthu, kupanga molondola, palibe kuwonjezera mankhwala. Limbikitsani mosamalitsa zowunikira zinayi zodziyendera, kuyang'anira patrol, kuyang'ana kwathunthu ndi kuyang'ana mwachisawawa, ndipo mtundu wake ndi wokhazikika.
Chithandizo chapamwamba: chithandizo TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment
TC mndandanda, oyenera chikhalidwe cha adherent maselo
Pogwiritsa ntchito chithandizo chapadera cha vacuum mpweya wa plasma, pamwamba pakhoza kupitiriza kunyamula mitundu iwiri yamagulu mofanana kwa nthawi yaitali, ndalama zabwino komanso zolakwika, kuonetsetsa kuti kutsata kwa selo kumakhala kofanana komanso kokhazikika. TC imakhala ndi kumatira bwino komanso kufalikira kuposa malo ofanana a TC akagwiritsidwa ntchito ngati ma cell endothelial, hepatocytes ndi chikhalidwe cha neuronal cell, ndipo amatha kukwaniritsa bwino kwambiri ntchito yama cell. Kukhutitsa chikhalidwe cha apamwamba mlingo adherent maselo.
TC Enhanced treatment Series, yoyenera chikhalidwe cha ma cell omwe ali ndi zofunika kwambiri
Advanced minofu chikhalidwe chithandizo, poyerekeza ndi muyezo TC mankhwala mankhwala, TC-wowonjezera pamwamba amatha kulimbikitsa selo adhesion ndi kutambasuka, mofulumira kukulitsa maselo, ndipo angagwiritsidwe ntchito kufalitsa wovuta maselo, monga maselo oyambirira kapena tcheru maselo, monga komanso ma cell omwe amakulira m'malo ocheperako (opanda seramu kapena kuchepa kwa seramu), kukulitsa kuchuluka kwa maselo omwe akuchulukirachulukira, kulimbikitsa kulumikizana kwa ma cell ndikuwonjezera, ndipo amatha kusungidwa kutentha kwachipinda ndi alumali yazaka ziwiri mapulogalamu.
Koposa-otsika kumanga mndandanda, oyenera chikhalidwe cha kuyimitsidwa maselo
Kupaka polima wapadera wa amphiphilic pamwamba pa chotengera cha chikhalidwe, chifukwa champhamvu kwambiri ya hydrophilicity ya pawiri iyi, mamolekyu amphiphilic amatha kuyamwa mamolekyu amadzi kuti apange khoma lamadzi, kotero kuti maselo, mamolekyu a protein ndi mabakiteriya sangathe kulumikizidwa ku chotengera cha chikhalidwe. , kotero ili ndi mawonekedwe a ultra-low cell adhesion.
Itha kubzalidwa poyimitsidwa kwa masiku opitilira 15. Pambuyo poyesedwa, chiwerengero cha maselo a AMA ultra-low adsorption ndi ochepera 2% mu chikhalidwe choyimitsidwa kwa masiku 21, omwe ali ofanana ndi zotsatira za mtundu wodziwika bwino wapadziko lonse.
Amagwiritsidwa ntchito kulima maselo amtundu wa fetal, maselo amagazi ndi maselo ena omwe amafunikira kukula mumayendedwe oyimitsidwa, ndipo amatha kulimbikitsa chikhalidwe cha maselo a 3D spheroid ndi organoids, ndipo ali ndi anti-adhesion properties kwa maselo amphamvu kwambiri.
Collagen mtundu l mankhwala pamwamba
Mtundu wa Collagen Woyamba umapezeka m'matenda ndi ziwalo zambiri, zomwe zimakhala ndi dermis, tendons ndi mafupa. Ndi puloteni yofunikira kwambiri yomwe imathandizira ma cell athunthu ndi minofu, komanso ndi gawo lofunikira pakukulitsa kukula kwa maselo. Chikhalidwe cha in vitro, collagen imagwira ntchito yofunika kwambiri pakumatira, morphogenesis, kukula, kusamuka komanso kusiyanitsa kwa maselo osiyanasiyana.
Mapulogalamu apadera ndi awa:
Imalimbikitsa kumamatira kwa ma cell ndi kutambasula
kuchulukirachulukira kwa ma cell
Chikhalidwe chopanda seramu kapena kuchepa kwa seramu
Cell Adhesion Assay
Kupititsa patsogolo kupulumuka kwa chikhalidwe cha ma cell
Nonpyrogenic, Nonendotoxicity, DNase/Rnase free, yosungidwa pa 2-8 ° C, ndi alumali moyo wa miyezi 6
Kutseketsa kwa radiation
Poly-D-lysine TACHIMATA pamwamba mankhwala
Poly-D-lysine (PDL) ndi mankhwala opangidwa omwe amatha kulimbikitsa kuyanjanitsa kwa selo ndi mapuloteni adsorption posintha mtengo wamtundu wa chikhalidwe. kupulumuka kwa ma cell ambiri apakati (CNS) zikhalidwe zoyambira zama cell.Popeza kuti PDL ndi molekyulu yopangidwa, sidzalimbikitsa ntchito zamoyo zama cell otukuka, komanso sizidzayambitsa zonyansa zotengedwa ndi ma polima achilengedwe.
Mapulogalamu apadera ndi awa:
Kumamatira ndi kukulitsa ma cell osiyanasiyana
Kusiyana kwa ma cell ndi kukula kwa neurite
Kumamatira kwa mizere yopatsirana mwachangu
Kuchulukitsa kupulumuka kwa zikhalidwe zoyambirira za neuronal
Chikhalidwe chopanda seramu kapena kuchepa kwa seramu
Malo osungira: Pamalo owuma pa 2 ° C mpaka 8 ° C, moyo wa alumali ndi zaka 2
Nthawi yotumiza: Jul-08-2023