Nzvimbo yekugadzira : kugadzirwa mu100,000-kirasi yakachena musangano
Kugadzira zvigadzirwa: yepamusoro-mhando polystyrene (GPPS)
Maitiro ekugadzira: Iyo chigadzirwa dhizaini yakanakisa, modhi yacho yakakwana, chaiyo kuumba, hapana kuwedzera kemikari. Nyatsoshandisa zviongorudzo zvina zvekuzviongorora, kuongorora patrol, kuongorora kwakazara uye kuongorora zvisina tsarukano, uye mhando yacho yakagadzikana.
Kurapa kwepamusoro: TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment
TC yakatevedzana, yakakodzera tsika yeadherent masero
Uchishandisa yakakosha vacuum gasi plasma kurapwa, iyo yepamusoro inogona kuramba ichitakura marudzi maviri emapoka zvakafanana kwenguva yakareba, chaji yakanaka uye mhosva isina kunaka, ive nechokwadi chekuti kutevedzera kwesero kwakafanana uye kwakagadzikana. TC ine kunamatira kuri nani uye kupararira pane yakafanana TC nzvimbo kana ichishandiswa endothelial masero, hepatocytes uye neuronal cell tsika, uye inogona kuwana yakanakisa sero adhesion performance. Kugutsa tsika dzepamusoro-soro adherent cells.
TC Enhanced yakarapwa Series, inokodzera sero tsika ine yakakwira yekutevedzera zvinodiwa
Advanced tishu tsika kurapwa, kana ichienzaniswa neyakajairwa TC-yakarapwa zvigadzirwa, TC-yakakwenenzverwa pamusoro inokwanisa kusimudzira kunamatira kwesero uye kuwedzera, nekukurumidza kuwedzera masero, uye inogona kushandiswa kuparadzira masero anodiwa, senge ekutanga maseru kana maseru anonzwisa tsitsi, se pamwe nemaseru akasimwa pasi pemamiriro ekukura akaganhurirwa (serum-isina kana serum-yakaderedzwa), wedzera kukurumidza kuwedzera kwesero, kukurudzira kusungirirwa kwesero uye kuwedzera, uye inogona kuchengetwa pakamuri tembiricha ine sherufu yemakore maviri uye yakafara siyana. applications.
Ultra-low binding series, inokodzera tsika yemasero ekumisa
Kupfekedza yakakosha amphiphilic polymer pamusoro peiyo tsika ngarava, nekuda kweiyo yakanyanya kusimba hydrophilicity yeiyi komputa, amphiphilic mamorekuru anogona kutora mamorekuru emvura kuti agadzire madziro emvura, kuitira kuti masero, mapuroteni mamorekuru uye mabhakitiriya haakwanise kusungirirwa kumudziyo wetsika. , saka ine maitiro e-ultra-low cell adhesion.
Inogona kusimudzwa mukumiswa kweanopfuura mazuva gumi nemashanu. Mushure mekuedzwa, iyo cell adhesion rate ye AMA ultra-low adsorption iri pasi pe2% mukusimwa tsika kwemazuva makumi maviri nerimwe, iyo yakafanana nemhedzisiro yemhando inozivikanwa yepasirese.
Inoshandiswa kurima fetal sphere masero, masero eropa uye mamwe maseru anoda kukura mukusimudzira tsika yepakati, uye anogona kusimudzira tsika ye3D spheroid masero uye organoids, uye ine anti-adhesion zvivakwa kune yakasimba viscous masero.
Collagen mhando l kurapwa kwepamusoro
Collagen Type I inowanikwa mune dzakawanda matishu uye nhengo, ine mazinga epamusoro anowanikwa mudermis, tendon uye mapfupa. Iyo ipuroteni yakakosha yezvimiro inotsigira maseru akazara uye matishu, uye zvakare yakakosha matrix yekuvandudza sero kukura. In in vitro tsika, collagen inoita basa rinokosha mukunamatira, morphogenesis, kukura, kutama uye kusiyanisa kwemasero akasiyana-siyana.
Zvakananga zvikumbiro zvinosanganisira:
Inosimudzira kunamatira kwesero uye kutambanudza
kukurumidza kuwedzera huwandu hwemasero
Serum-isina kana serum-yakaderedzwa tsika
Cell Adhesion Assay
Kuvandudza mararamiro ekutanga masero
Nonpyrogenic, Nonendotoxicity, DNase/Rnase yemahara, yakachengetwa pa2-8°C, ine sherufu yeupenyu hwemwedzi mitanhatu.
Irradiation sterilization
Poly-D-lysine coated pamusoro kurapwa
Poly-D-lysine (PDL) inhengo yekugadzira iyo inogona kukurudzira kunamatira kwesero uye protein adsorption nekushandura iyo yepamusoro chargeji yetsika substrate.Kuwedzera pakusimudzira masero adhesion, poly-D-lysine-yakagadziriswa nzvimbo inotsigira neurite kubuda uye kuvandudza. kupona kweakawanda central central nervous system (CNS) primary cell cultures.Sezvo PDL iri molecule yekugadzira, haizosimudziri basa rehupenyu hwemasero akagadzirwa, uye haizounzi tsvina inotakurwa nemapolima echisikigo.
Zvakananga zvikumbiro zvinosanganisira:
Kunamatira uye kuwedzera kwemhando dzakasiyana dzemasero
Kusiyanisa kwesero uye neurite kubuda
Kunamatira kwe fastidious transfected cell mitsara
Kuwedzera kupona kwekutanga neuronal tsika
Serum-isina kana serum-yakaderedzwa tsika
Mamiriro ekuchengetedza: Pasi pemamiriro akaoma pa 2 ° C kusvika 8 ° C, mararamiro esherufu makore maviri
Nguva yekutumira: Jul-08-2023