Iimveliso zeNkcubeko yeSeli

Indawo yokuvelisa:  imveliso kwi-100,000-class workshop ecocekileyo

Imathiriyeli ekrwada yokuvelisa:  ipolystyrene ekumgangatho ophezulu (GPPS)

Inkqubo yokuvelisa:  Uyilo lwemveliso lugqwesileyo, imodeli igqityiwe, iyenza ngokuchanekileyo, akukho kongeza kweekhemikhali. Ukuphumeza ngokungqongqo ukuhlolwa okune kokuzihlola, ukuhlolwa kwe-patrol, ukuhlolwa okupheleleyo kunye nokuhlolwa okungahleliwe, kwaye umgangatho uzinzile.

Unyango olungaphezulu:  TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment

Uchungechunge lwe-TC, olulungele inkcubeko yeeseli ezibambelelayo

Ukusebenzisa unyango olukhethekileyo lwe-vacuum yegesi yeplasma, umphezulu unokuqhubeka nokuthwala iindidi ezimbini zamaqela ngokufanayo ixesha elide, intlawulo efanelekileyo kunye nentlawulo engafanelekanga, qinisekisa ukuba ukuthotyelwa kweseli kufana kakhulu kwaye kuzinzile.Ukuqaliswa kweentlawulo ezimbini kwenza umphezulu weJindian I-TC ine-adhesion engcono kunye nokusabalalisa kuneendawo ezifanayo ze-TC xa zisetyenziselwa iiseli ze-endothelial, i-hepatocytes kunye nenkcubeko ye-neuronal cell, kwaye inokufezekisa ukusebenza kakuhle kwe-cell adhesion. Ukwanelisa inkcubeko yenqanaba eliphezulu lokubambelela kwiiseli.

I-TC Enhanced treatment Series, ilungele inkcubeko yeseli eneemfuno eziphezulu zokubambelela

Unyango oluphezulu lwenkcubeko yezicubu, xa kuthelekiswa neemveliso eziqhelekileyo eziphathwe nge-TC, umphezulu ophuculweyo we-TC unamandla okukhuthaza ukunamathela kunye nokwandiswa kweeseli, ukwandisa ngokukhawuleza inani leeseli, kwaye ingasetyenziselwa ukusasaza iiseli eziyimfuneko, ezinjengeeseli eziphambili okanye iiseli ezibuthathaka, njengoko kunye neeseli ezikhuliswe phantsi kweemeko zokukhula ezithintelweyo (i-serum-free okanye i-serum-incitshisiwe), ukwandisa ukwanda ngokukhawuleza kwabemi beeseli, ukukhuthaza ukudibanisa kweeseli kunye nokwandiswa, kwaye inokugcinwa kwiqondo lokushisa kunye ne-shelf yeminyaka emi-2 kunye noluhlu olubanzi izicelo.

I-Ultra-low binding series, ifanelekile kwinkcubeko yeeseli zokunqunyanyiswa

Ukugquma i-amphiphilic polymer ekhethekileyo kumphezulu wenqanawa yenkcubeko, ngenxa ye-hydrophilicity eyomeleleyo yale khompawundi, iimolekyuli ze-amphiphilic zinokufunxa iimolekyuli zamanzi zenze udonga lwamanzi, ukuze iiseli, iimolekyuli zeprotheyini kunye neebhaktheriya zingakwazi ukuncamathela kwinqanawa yenkcubeko. , ngoko ke ineempawu ze-ultra-low adhesion cell.

Ingakhuliswa ngokunqunyanyiswa ngaphezu kweentsuku ezili-15. Emva kokuvavanya, izinga lokubambelela kwiseli ye-AMA ye-adsorption ye-ultra-low ingaphantsi kwe-2% kwinkcubeko yokunqunyanyiswa kweentsuku ze-21, efana nesiphumo se-brand eyaziwayo ngamazwe ngamazwe. 

Isetyenziselwa ukukhulisa iiseli ze-fetal sphere, iiseli zegazi kunye nezinye iiseli ezifuna ukukhula kwindawo yenkcubeko yokunqunyanyiswa, kwaye inokukhuthaza inkcubeko yeeseli ze-spheroid ze-3D kunye ne-organoids, kwaye ineempawu ezichasene nokunamathela kwiiseli ze-viscous ezinamandla.

Collagen uhlobo l unyango umphezulu

Uhlobo lwe-Collagen I lufumaneka kwizicubu ezininzi kunye namalungu, kunye namanqanaba aphezulu afunyenwe kwidermis, i-tendon kunye namathambo. Yiprotheni eyimfuneko yolwakhiwo exhasa iiseli ezipheleleyo kunye nezicubu, kwaye ikwayimatrix ebalulekileyo yokuphucula ukukhula kweeseli. Kwinkcubeko ye-in vitro, i-collagen idlala indima ebalulekileyo kwi-adhesion, i-morphogenesis, ukukhula, ukufuduka kunye nokuhlukana kweeseli ezahlukeneyo.

Usetyenziso oluthile lubandakanya:

Ikhuthaza ukunamathela kweeseli kunye nokwelula

ukwanda ngokukhawuleza kwabemi beeseli

I-serum-free okanye inkcubeko yokunciphisa i-serum

Uvavanyo lokuncamathela kwiseli

Ukuphucula ukusinda kwenkcubeko yeeseli eziphambili

I-Nonpyrogenic, i-Nonendotoxicity, i-DNase / i-Rnase ikhululekile, igcinwe kwi-2-8 ° C, kunye ne-shelf yokuphila kweenyanga ze-6

Ukuvala inzala kwimitha

I-Poly-D-lysine igqunywe ngonyango lomphezulu

I-Poly-D-lysine (i-PDL) i-synthetic compound enokukhuthaza ukunamathela kwi-cell kunye ne-protein adsorption ngokuguqula umrhumo womphezulu we-substrate yenkcubeko.Ukongezelela ekukhuthazeni ukunamathela kweeseli, i-poly-D-lysine-treated surfaces isekela ukuphuma kwe-neurite kunye nokuphucula. ukusinda kwezinto ezininzi ze-nervous system (CNS) eziphambili zeenkcubeko zeeseli.Njengoko i-PDL i-molecule ye-synthetic, ayiyi kuvuselela umsebenzi we-biological weeseli ezikhuliswe, kwaye ayiyi kwazisa ukungcola okwenziwa yi-polymers yendalo.

Usetyenziso oluthile lubandakanya:

Ukunamathela kunye nokwandiswa kweeseli ezahlukeneyo

Ukwahlulahlula kweeseli kunye nokukhula kwe-neurite

Ukuncamathela kwemigca yeeseli ezosulelwe ngokukhawuleza

Ukwanda kokusinda kweenkcubeko eziphambili ze-neuronal

I-serum-free okanye inkcubeko yokunciphisa i-serum

Iimeko zokugcina: Ngaphantsi kweemeko ezomileyo kwi-2 ° C ukuya kwi-8 ° C, ubomi beshelufu yiminyaka emi-2