Indawo yokukhiqiza: ukukhiqizwa endaweni yokusebenzela ehlanzekile yekilasi eyi-100,000
Izinto zokusetshenziswa zokukhiqiza: i-polystyrene yekhwalithi ephezulu (GPPS)
Inqubo yokukhiqiza: Idizayini yomkhiqizo inhle, imodeli iphelele, yakha ngokunemba, akukho ukwengeza kwamakhemikhali. Sebenzisa ngokuqinile ukuhlola okune kokuzihlola wena, ukuhlola i-patrol, ukuhlola okugcwele nokuhlola okungahleliwe, futhi ikhwalithi izinzile.
Ukwelashwa okungaphezulu: TC treated ,TC Enhanced treated, Ultra-low binding treatment, Collagen l surface treatment, Poly-D-lysine coated surface treatment
Uchungechunge lwe-TC, olufanele isiko lamaseli anamathelayo
Ngokusebenzisa ukwelashwa okukhethekile kwe-vacuum gas plasma, indawo engaphezulu ingaqhubeka nokuthwala izinhlobo ezimbili zamaqembu ngokufanayo isikhathi eside, ukushaja okuhle kanye nokushaja okungekuhle, qinisekisa ukuthi ukubambelela kweseli kufana kakhulu futhi kuzinzile.Ukwethulwa kwamashaji aphindwe kabili kwenza ubuso beJindian I-TC inokunamathela kangcono nokusabalalisa kunezindawo ezifanayo ze-TC lapho isetshenziselwa amaseli e-endothelial, ama-hepatocyte kanye nesiko lamaseli e-neuronal, futhi ingafinyelela ukusebenza okuhle kakhulu kokunamathela kwamaseli. Ukwanelisa isiko lamaseli anamathelayo ezingeni eliphezulu.
I-TC Enhanced treatment Series, ifanele isiko lamaseli anezidingo zokubambelela okuphezulu
Ukwelashwa okuthuthukisiwe kwesiko lezicubu, uma kuqhathaniswa nemikhiqizo ejwayelekile elashwe yi-TC, indawo ethuthukisiwe ye-TC inamandla okukhuthaza ukunamathela kweseli nokunwetshwa, ukwandisa ngokushesha inani lamangqamuzana, futhi ingasetshenziswa ukusabalalisa amaseli afuna ngamandla, njengamaseli ayinhloko noma amaseli azwelayo, kanye namaseli akhuliswe ngaphansi kwezimo zokukhula ezikhawulelwe (angenayo i-serum noma ancishisiwe), andise inani lamaseli akhula ngokushesha, akhuthaze ukunamathiselwa kwamangqamuzana nokunwetshwa, futhi angagcinwa ekamelweni lokushisa elihlala eshalofini leminyaka engu-2 kanye nohlu olubanzi lwamaseli. izicelo.
Uchungechunge olubophezelayo oluphansi kakhulu , lufanele isiko lamaseli amisiwe
Ukumboza i-polymer ekhethekile ye-amphiphilic phezu komkhumbi wamasiko, ngenxa ye-hydrophilicity eqinile yale nhlanganisela, ama-amphiphilic molecule angakwazi ukumunca ama-molecule wamanzi ukuze akhe udonga lwamanzi, ukuze amangqamuzana, ama-protein molecule nama-bacterium angakwazi ukuxhunywa emkhunjini wamasiko. , ngakho-ke inezici zokunamathela kweseli eliphansi kakhulu.
Ingakhuliswa ngokumiswa isikhathi esingaphezu kwezinsuku eziyi-15. Ngemuva kokuhlolwa, izinga lokunamathela kweseli le-AMA ultra-low adsorption lingaphansi kuka-2% esikweni lokumiswa izinsuku ezingama-21, elifana nomphumela womkhiqizo owaziwayo wamazwe ngamazwe.
Isetshenziselwa ukuhlakulela amangqamuzana e-fetus sphere, amangqamuzana ezinhlayiya zegazi namanye amaseli adinga ukukhula endaweni yesiko lokumiswa, futhi angakhuthaza isiko lamaseli e-3D spheroid kanye nama-organoid, futhi anezindawo zokulwa nokunamathela kumaseli abonakalayo anamandla.
Uhlobo lwe-Collagen l ukwelashwa kwendawo
I-Collagen Type I itholakala ezicutshini eziningi nasezithweni, ezinamazinga aphezulu kakhulu atholakala ku-dermis, ama-tendon namathambo. Kuyiprotheyini yesakhiwo ebaluleke kakhulu esekela amangqamuzana aphelele nezicubu, futhi iyi-matrix ebalulekile yokuthuthukisa ukukhula kwamangqamuzana. In vitro isiko, collagen idlala indima ebalulekile adhesion, morphogenesis, ukukhula, ukufuduka kanye nokuhlukaniswa kwamaseli ahlukahlukene.
Izinhlelo zokusebenza ezithize zifaka:
Ikhuthaza ukunamathela kwamaseli nokwelula
ukwanda ngokushesha kwamaseli abantu
Isiko elingenayo i-serum noma elehlisiwe
I-Cell Adhesion Assay
Thuthukisa ukuphila kwesiko lamaseli
I-Nonpyrogenic, Nonendotoxicity, DNase/Rnase free, igcinwe ku-2-8°C, nempilo yeshelufu yezinyanga eziyi-6
Ukuvala inzalo ngemisebe
I-Poly-D-lysine coated surface treatment
I-Poly-D-lysine (PDL) iyinhlanganisela yokwenziwa engakhuthaza ukunamathela kwamaseli kanye nokukhangiswa kwamaprotheni ngokushintsha ukushaja okungaphezulu kwe-culture substrate.Ngaphezu kokukhuthaza ukunamathela kwamangqamuzana, izindawo ezilashwe nge-poly-D-lysine zisekela ukuphuma kwe-neurite futhi zithuthuke. ukusinda kwamasiko amaningi esimiso sezinzwa eziyinhloko (CNS).Njengoba i-PDL iyi-molecule yokwenziwa, ngeke ishukumise umsebenzi webhayoloji wamangqamuzana akhulisiwe, futhi ngeke ize yethule ukungcola okwenziwa ama-polymer emvelo.
Izinhlelo zokusebenza ezithize zifaka:
Ukunamathela kanye nokwandiswa kwamaseli ahlukahlukene
Ukuhlukaniswa kwamaseli kanye nokukhula kwe-neurite
Ukunamathela kolayini beselula abasulelekile ngokushesha
Ukwanda kokusinda kwamasiko ayisisekelo e-neuronal
Isiko elingenayo i-serum noma elehlisiwe
Izimo zokugcina: Ngaphansi kwezimo ezomile ku-2°C kuya ku-8°C, isikhathi seshelufu siyiminyaka emi-2
Isikhathi sokuthumela: Jul-08-2023