I-UV Assay Microplates
Ipuleti le-UV livumela u-80%, 260nm/280nm ukukhanya kwegagasi kwe-UV ukuthi kudlule esixhumi esibonakalayo esiphansi esiphansi, kusetshenziswa lesi sici ukuze kulinganise okuqukethwe nokuhlanzeka kwe-deoxyribonucleic acid (DNA) kanye namaprotheni (Amaprotheni)
Imininingwane: Ipuleti le-UV eliphansi elingu-96 elicace kahle, amapuleti e-UV angama-96 amnyama anephansi elicacile, amapuleti e-UV aphansi angama-384 acace kahle, angama-384 amapuleti e-UV amnyama anephansi elicacile
Umkhiqizo ngokuvamile awufaki ikhava yepuleti (ingafaniswa uma kudingeka)
Indlela i-UV Plate elinganisa ngayo i-DNA ne-Protein okuqukethwe kumasampuli:
Uma ukukhanya kwe-ultraviolet kwamaza amafushane angu-260nm kukhanyisa isampula ye-DNA, izokhangiswa ngama-molecule e-DNA, futhi ukudluliswa kokukhanya kwe-ultraviolet kuzokwehla. Ngokuphambene, inani le-optical density (Optical Density) elilinganiswa nge-spectrophotometer lizokhuphuka ngokungafani. Usayizi wevelu yokuminyana bungabalwa ngohlelo lwesofthiwe ukuze kubalwe okuqukethwe kwangempela kwe-DNA. Lapho ukukhanya kwe-ultraviolet kwamaza amafushane angu-280nm kukhanyisa isampula yephrotheni, izomuncwa ama-molecule amaprotheni.
Ngaphezu kwalokho, ukudluliswa kokukhanya kwe-ultraviolet kuyehla, ngokuphambene nalokho, inani le-optical density (Optical Density) elilinganiswa nge-spectrophotometer lizokhuphuka ngokuphambene, futhi okuqukethwe kwangempela kwe-DNA kungabalwa ngohlelo lwesofthiwe ngevelu etholiwe yokuminyana kokubona. .
Ipuleti elicacile elijwayelekile elingu-96 lenziwe nge-PSˎPCˎ noma i-PET. Lapho umthombo wokukhanya udlula esihlungini esingu-260nm futhi ugongolo oluhloliwe lukhanyiselwa kusixhumi esibonakalayo se-PSˎPCˎ noma se-PET, ukukhanya kwe-ultraviolet kwamaza amafushane kudonswa okuphakathi futhi akukwazi ukudlula esibonakalayo, ngakho-ke ngeke kusetshenziswe njengepuleti lokukhanya le-UV. ukusebenzisa.
96 ipuleti le-UV eliphansi elicace kahle
96 ipuleti le-UV elimnyama elinephansi elicacile
384 ipuleti le-UV eliphansi elicace kahle
384 ipuleti le-UV elimnyama elinephansi elicacile
Isikhathi sokuthumela: Nov-11-2022