ʻO nā Microplates hōʻike UV
Hiki i ka pā UV ke hiki i ka 80%, 260nm/280nm wavelength UV kukui e hele i loko o kāna kikowaena palahalaha, me ka hoʻohana ʻana i kēia hiʻohiʻona e helu i ka ʻike a me ka maʻemaʻe o ka deoxyribonucleic acid (DNA) a me ka protein (Protein)
Nā ʻōlelo kikoʻī: 96 pāpaʻa palahalaha maikaʻi ma lalo o ka pā UV, 96 pāpaʻa UV ʻeleʻele maikaʻi me ka ʻaoʻao akaka, 384 pāpaʻa UV palahalaha maikaʻi ma lalo, 384 pāpaʻa UV ʻeleʻele maikaʻi me lalo akaka.
ʻAʻole hoʻokomo ka huahana i ka uhi pā (hiki ke hoʻohālikelike ʻia ma ke koi)
Pehea ʻo UV Plate e helu ai i ka maʻiʻo DNA a me ka Protein i nā laʻana:
Ke hoʻomālamalama ka 260nm pōkole pōkole ultraviolet i ka DNA sample, e hoʻopili ʻia e nā molekala DNA, a e emi ka transmittance o ke kukui ultraviolet. ʻO ka mea ʻē aʻe, e hoʻonui ʻia ke ʻano like ʻole o ka waiwai mānoanoa optical (Optical Density) i ana ʻia e ka spectrophotometer. Hiki ke helu ʻia ka nui o ka waiwai nui e ka polokalamu polokalamu e helu ai i ka ʻike maoli o DNA. Ke hoʻomālamalama ʻo 280nm pōkole pōkole ultraviolet i ka hāpana protein, e hoʻopili ʻia ia e nā molekole protein.
Eia kekahi, ke emi nei ka transmittance o ke kukui ultraviolet, ma ka mea ʻē aʻe, ʻo ka optical density waiwai (Optical Density) i ana ʻia e ka spectrophotometer e hoʻonui i ka ʻokoʻa, a ʻo ka ʻike maoli o ka DNA hiki ke helu ʻia e ka polokalamu lako polokalamu e ka waiwai o ka optical density. .
Hana ʻia ka pā maʻemaʻe 96-well me PSˎPCˎ a i ʻole PET. Ke hele ke kumu kukui i ka kānana 260nm a ua hoʻomālamalama ʻia ka lāʻau pale i ka PSˎPCˎ a i ʻole PET mea pānaʻi, hoʻopili ʻia ke kukui ultraviolet pōkole pōkole e ka mea waena a ʻaʻole hiki ke hele i loko o ka interface, no laila ʻaʻole hiki ke hoʻohana ʻia e like me ka pā kukui UV. hoʻohana.
96 maikaʻi maʻemaʻe pālahalaha lalo UV pā
96 maikaʻi ʻeleʻele UV pā me ka maopopo lalo
384 maikaʻi maʻemaʻe pālahalaha lalo UV pā
384 maikaʻi ʻeleʻele UV pā me ka maopopo lalo
Ka manawa hoʻouna: Nov-11-2022